【摘要】 采用随机扩增多态DNA(RAPD)技术对取自六安、龙感湖、淮南和高淳等4个地理群体的日本沼虾(Macrobrachium nipponense)各30个个体进行遗传多样性分析。在事先优化的反应条件下,所使用的OPP、OPX2个系列40个随机引物中,有16个引物扩增出清晰稳定的片断,共产生173个位点,大小在250~2 000 bp,利用PopGen 1.32进行分析,日本沼虾多态位点为75个(多态性片断的比例为43.4%),Shan-non信息指数为0.177 1~0.246 6。遗传距离显示日本沼虾群体间有一定遗传分化,其中高淳群体和龙感湖群体之间遗传距离最大(0.171 2),六安群体和淮南群体之间最小(0.114 6)。用UPGMA对4个地理群体进行聚类分析,六安和淮南群体首先聚在一起,高淳和龙感湖群体最后。更多还原

【Abstract】 Random amplified polymorphic DNA(RAPD) technique was applied to investigate the genetic diversity among 4 geographic Macrobrachium nipponense,which were collected from Liu’an(Anhui),Longganhu Lake(Anhui),Huainan(Anhui) and Gaochun(Jiangsu) respectively.Under predetermined optimal reaction conditions,16 random primers were employed to scan the genomic DNA in 30 individuals of each of 4 geographic stocks,and a total of 173 reproducible RAPD markers with a molecular of weight ranged from 250~2 000 bp.The experimental data were analyzed with PopGen 1.32.As analyzed,the proportions of polymorphic loci of Macrobrachium nipponense was 43.4%,and the Shannon index varied from 0.177 1 to 0.246 6 among stocks.The genetic distance revealed that the differentiation had taken place to some extend among the Macmbrachium nipponensis.The maximum genetic distance occurred between the stock of Gaochun and Longganhu populations,and the minimum between Huainan and Liu’an populations.Clustering of the stocks was analyzed by UPGMA,and the results showed the populations of Liu’an and Huainan assembled one branch first,the last did Gaochun and Longganhu.更多还原