【摘要】 目的探讨大鼠死后不同时间肾、脾3-磷酸甘油醛脱氢酶(GAPDH)mRNA的表达对死亡时间(PMI)推断的应用价值。方法28只大鼠断颈处死后置于20℃温控系统内,分别于死后0,8,16,24,32,40,48h共7个时相点(n=4)解剖,利用荧光RT-PCR技术检测大鼠肾、脾GAPDHmRNA相对表达量的变化。结果在大鼠死后40h肾、32h脾内可检测出GAPDHmRNA,且其表达产物均呈规律性下降趋势(荧光强度逐渐变暗并最终消失)。结论检测大鼠死后不同时间肾、脾GAPDHmRNA的表达对PMI推断具有一定的应用价值。更多还原

【Abstract】 Objective To study changes in expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in the kidney and spleen of dead rats and its application in deducing the postmortem intervals (PMI), so as to look for a new method for PMI deduction. Method 28 rats were killed by cervical dislocation, placed in a chamber with temperature-controlling system at 20℃ for 0 to 48h postmortem, and autopsied at 0, 8, 16, 24, 32, 40 and 48h respectively (n=4 for each time point). The expression of GAPDH mRNA in kidney and spleen was assessed with fluorescence semi-quantitative RT-PCR. Results The amplified products of GAPDH mRNA could be detected in kidney within 40h and in spleen within 32h postmortem respectively, and they showed a decreasing tendency as its fluorescence intensity declined gradually and lost at last. Conclusion Detection of GAPDH mRNA expression in kidney and spleen of rats at different periods after death by fluorescence semi-quantitative RT-PCR seems to be of some value for estimation of PMI.更多还原