Objective To explore the relationship between pain of prostatitis and activation of glia cells in spinal cord. Methods To investigate the activation of astroglia cells and microglia cells in spinal cord after irritation of the prostate of the rats with complete Freund′s adjuvant (CFA) at different time using immunohistochemical staining. Results Activation of astroglia cells and microglia cells were significantly increased in deeper laminae and superficial laminae of the dorsal horn of L_6-S_1 spinal cord o...