【摘要】 目的探讨核因子(NF-κB)抑制剂MG-132对支气管上皮细胞释放细胞因子白细胞介素(IL)-6的影响。方法人支气管上皮细胞系BEAS-2B细胞与MG-132(10μmol·L-1)共同培养12h,细胞核中NF-κB活性和细胞培养上清液中IL-6浓度用酶联免疫吸附试验(ELISA)方法定量,BEAS-2B细胞总IL-6mRNA表达,应用逆转录聚合酶链反应(RT-PCR)分析。结果BEAS-2B细胞与MG-132共同培养过程中,BEAS-2B细胞IL-6mRNA上调,细胞培养上清液中IL-6释放量显著增加,而且IL-6增加量与细胞培养液中MG-132浓度呈正相关,但与NF-κB活性无关。结论MG-132可诱导BEAS-2B细胞释放IL-6,但不是通过NF-κB活化途径。更多还原

【Abstract】 Aim To investigate the effect of MG-132,an inhibitor of nuclear factor-κB (NF-κB) on IL-6 release from BEAS-2B cell,a human bronchial epithelial cell line.Methods BEAS-2B cells were cultured with 10μmol·L~ -1 MG-132 for 12h;IL-6 mRNA expression in BEAS-2B cells was analyzed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR);NF-κB activity in the nuclei of BEAS-2B cells and IL-6 concentration release in cell culture supernatant were analyzed by Enzyme-Linked ImmunoSorbent Assay (ELISA).Results IL-6 mRNA expression in BEAS-2B cells was upregulated markedly and IL-6 release was elevated significantly in culture supernatant in BEAS-2B cells cultured with MG-132.Conclusion BEAS-2B cells can be activated by NF-κB inhibitor,MG-132,to release IL-6.更多还原