【摘要】 目的观察选择性COX-2抑制剂尼美舒利对食管癌Eca-109细胞增殖、凋亡及COX-2表达的影响,并探讨与抑癌基因P27kip1的关系。方法用MTT法测定Eca-109细胞增殖;透射电镜及流式细胞仪观察细胞凋亡及细胞周期;RT-PCR法检测Eca-109细胞COX-2mRNA表达变化;Westernblot检测COX-2和P27kip1蛋白表达变化。结果尼美舒利以时间、剂量依赖方式抑制Eca-109增殖,增加G0/G1期细胞比例;呈剂量依赖性诱导细胞凋亡,降低COX-2mRNA和蛋白表达,上调P27kip1蛋白表达。结论尼美舒利可抑制食管癌Eca-109细胞增殖,使细胞周期受阻于G0/G1期并诱导细胞凋亡。其机制可能是通过下调COX-2表达和上调P27kip1蛋白表达水平实现的。更多还原

【Abstract】 Objective To investigate the effects of selective COX-2 inhibitor nimesulide on growth inhibition,apoptosis and expression of COX-2 of human esophageal carcinoma Eca-109 cell line; and analyzed the correlation with the anti-oncogene,P277^kip1. Methods MTT assay was used to detect the proliferation of Eca-109 cell. Apoptosis and cell cycle were determined by electronic microscopy and flow cytometry. The expression of COX-2 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）,the protein expression of COX-2 and P277^kip1 were examined by Western blot analysis. Results Nimesulide significantly inhibited the proliferation of Eca-109 cell line in a time-and dose-depenent fashion; increased the proportion of cells in the G₀/G₁ phase and induced apoptosis of the cells in a dose-dependent（manner）. Meanwhile,nimesulide can down-regulated the expression of COX-2 and up-regulated the expression of P277^kip1 protein.Conclusion Nimesulide can inhibit the proliferation of Eca-109 cells,cause G₀/G₁ phase cell cycle arrest and induce apoptosis.The mechanism is probably explained with down-regulation of the expression of COX-2 and up-regulation of P277^kip1 expression.更多还原