【摘要】 实验选用唐古特大黄(Rheum tanguticum M axim.ex Regel.)种子萌发的无菌苗为外植体进行离体培养。结果表明,2mg/L的2,4-D最有利于愈伤组织的形成,但更高浓度的2,4-D会影响诱导效果;1mg/L的6-BA有利于愈伤组织的快速诱导,但单独的6-BA无诱导作用。更多还原

【Abstract】 Seed germinated seedlings of Rheum tanguticum Maxim.ex Regel.were used as explants cultured in vitro to induce the callus.The experimental results showed that 2mg/L 2,4-D was beneficial to the formation of callus,but higher concentration of 2,4-D had bad effect on callus induction.1mg/L 6-BA could accelerate the growth of the callus in the medium with 2,4-D,while 6-BA couldn’t induce callus itself.更多还原