【摘要】 设计了1种从油菜种子中分离高质量总RNA的方法,有效的排除了种子中富含的多酚、多糖和蛋白质等杂质的污染。分离的总RNA经甲醛变性凝胶电泳和紫外光检测,可见28S和18S rRNA的2条清晰的带型,完整无降解,其A260/A280在1.8~2.0之间。研究结果证明,利用本方法提取的油菜种子总RNA具有很高的质量和纯度,且得率高,完全能满足后续的RT-PCR、全长基因的克隆和Northern blotting分析等分子生物学研究。更多还原

【Abstract】 A highly efficient method of extracting total RNA from rapeseed was developed.Polyphenols,polysaccharides and protein,rich in rapeseed cells,could be removed effectively.Two clear bands of 28S rRNA and 18S rRNA isolated from rapeseeds could be detected by formaldehyde denaturalization agarose gel electrophoresis.It was proved that RNA was intact without degradation.The ratio of A260/A280 is 1.8~2.0.The results showed that the RNA obtained from rapeseed with this method had high purity and quality and could be used in molecular biological research,like RT-PCR,full-length cDNA cloning and Northern blotting analysis directly.更多还原