【摘要】 根据丝氨酸/苏氨酸蛋白激酶类催化结构域Ⅰ和Ⅸ的氨基酸保守序列设计简并引物,PCR扩增‘紫花芒’杧果嫩绿色叶片基因组DNA。在NCBI中用BlastX比对发现,有6个阳性克隆是抗病基因同源序列,并命名为PK1~PK6,GenBank注册序列号为AY693369~AY693371和AY776277~AY776279。在PK3、PK4和PK6中发现了丝氨酸/苏氨酸蛋白激酶的9个催化结构域Ⅰ~Ⅸ,而其它3个克隆具有部分结构域。氨基酸序列同源性分析还发现,6个克隆编码的氨基酸序列都与受体样蛋白激酶有较高的氨基酸序列同源性。系统进化树分析还表明它们都是可能的抗病基因同源序列。总之,6个克隆不仅是抗病基因同源序列而且是可能的受体样蛋白激酶基因同源序列。更多还原

【Abstract】 Based on the conserved amino acid sequences of the catalytic domainⅠand Ⅸ of the Serine/Threonine(STK)protein kinase-like proteins,the degenerated primers were designed and PCR was performed on genomic DNA extracted from the peak-green leaf of mango(M.indica cv.’Zihuamang’) and six DNA fragments of 500 bp were cloned and confirmed as disease resistance gene analogs(RGAs) coding STK protein kinase-like proteins after aligned in the GenBank with Blast X.They were named as PK1-PK6 with the accession number of AY693369-AY693371 and AY776277-AY776279 in the Genbank.All catalytic domainsⅠ-Ⅸ of STK protein kinase were found in the translations of PK3,PK4 and PK6.The six clones were also found as disease resistance gene analogs by molecular phylogenic tree analysis.All of their deduced amino acids highly matched with the receptor-like protein kinase via Blast X in NCBI.It showed that the six clones were the disease resistance gene analogs and the probable receptor-like kinase gene analogs.更多还原