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阿牙克库木湖嗜盐菌的分离及功能酶的筛选

Isolation and enzyme screening of halophiles from Ayakekum Lake.

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【作者】 孟凡旭吴敏张会斌童浩萱殷佳慧

【Author】 MENG Fan-xu~1,WU Min~1,ZHANG Hui-bin~2,TONG Hao-xuan~1,YIN Jia-hui~1(1.College of Life Science,Zhejiang University,Hangzhou 310027,China;2.Altun Mountain National Nature Reserve,Kuerle 841000,China)

【机构】 浙江大学生命科学学院阿尔金山国家级自然保护区管理处浙江大学生命科学学院 浙江杭州310027浙江杭州310027新疆库尔勒841000

【摘要】 从新疆阿尔金山国家自然保护区阿牙克库木湖采集的泥样和水样中,分离培养了90株嗜盐菌,其中多数为中度和极端嗜盐菌,颜色以红色和白色为主,细胞形态多为杆状、球状,97%呈革兰氏阴性,研究结果表明该湖有着丰富的嗜盐微生物资源.对分离菌株进行了几种重要工业用酶的筛选,发现其中淀粉酶产生菌有4株,菌株AJ225和AJ243淀粉酶活性较高;蛋白酶产生菌有7株;脂肪酶生产菌有13株,菌株AJ238和AJ265具有较高的酶产量;在分离菌株中未检测到纤维素酶和木聚糖酶活性.

【Abstract】 Mud and water samples were obtained from Ayakekum Lake located in Altun Mountain National Reserve of Xinjiang Uygur Autonomous Region,and ninety halopholic strains were isolated.Most of isolates were moderately or extremely Halophilic.The number of red and white colonies is greater than that of colonies with other coloures.The cells of the isolates were mostly rod or cocci shaped,and approximately 97% percent of the strains was Gram-negative.It was indicated that there are abundant resource of halophilic microorganism in Ayakekum Lake.Industrially important enzymes from these strains were screened subsequently.The result showed that four strains produce amylase,among which the strain AJ225 and strain AJ243 posses high enzyme productivity.Seven strains produced proteinase.Lipase is produced by thirteen strains,and two of them,strain AJ238 and AJ265,are high productive strains.Neither cellulase nor xylanase enzyme activity was detected among these strains.

【关键词】 嗜盐菌阿牙克库木湖功能酶
【Key words】 HalophilesAyakekum Lakeenzyme
【基金】 国家自然科学基金资助项目(30370029);国家重点基础研究发展规划资助项目(973计划)子项目(2004CB719604-3)
  • 【文献出处】 浙江大学学报(理学版) ,Journal of Zhejiang University(Science Edition) , 编辑部邮箱 ,2006年06期
  • 【分类号】Q93-3
  • 【被引频次】23
  • 【下载频次】466
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