【摘要】 采用PCR和DNA测序技术,对杭州与绍兴产桃花水母标本的核糖体小亚基rRNA基因进行了扩增与测序.经序列比对分析,杭州与绍兴产桃花水母的核糖体小亚基rRNA基因序列与已知索氏桃花水母的序列极为相似,相似度分别为99.61%和99.45%;与索氏桃花水母的遗传距离均为0.001;经分子系统树分析,杭州与绍兴产桃花水母与索氏桃花水母的分支置信度高达100%.综上分析,杭州与绍兴产桃花水母均为索氏桃花水母(Craspe-dacusta sowerbyi),该结论与其形态学分类相一致.更多还原

【Abstract】 The small subunit ribosomal RNA genes of Craspedacusta sp.from Hangzhou and Shaoxing were proliferated and sequenced.As a result of sequence analyses,the small subunit ribosomal RNA gene sequences of C.sp.from Hangzhou and Shaoxing are most similar to that of C.sowerbyi and the similarity percent are(99.61%) and(99.45%)respectively.The pairwise genetic distances between C.sp.from Hangzhou or Shaoxing and C.sowerbyi are both 0.001.The molecular phylogenetic tree shows 100% degree of confidence of branch between C.sp.of Hangzhou,Shaoxing,and C.sowerbyi. Summarily,C.sp.from Hangzhou and Shaoxing are C.sowerbyi.The conclusions are consistent with the results of morphological taxonomy.更多还原