【摘要】 采用MTT和SRB法研究了双核铂(Ⅱ)配合物[{Pt(H2O)2I}-μOOC-COO-{Pt(H2O)2I}](BPI)对人体癌细胞的增殖抑制作用,又通过流式细胞法、Giemsa染色法、等离子体质谱法研究了它的抗癌机制.实验结果表明:在10μmol.L-1浓度下,该配合物对HL-60,BGC-823,Bel-7402,KB,Hela,HCT-8,MCF-7和EJ 8种肿瘤细胞都表现出有较高的活性,对HL-60、BGC-823和Bel-7402 3种肿瘤细胞的抑制率都在70%以上,它能阻止HL-60细胞G2+M→G1期的进行;对HL-60细胞的凋亡诱导作用不明显;在相同浓度情况下其与HL-60细胞的DNA键合量大于顺铂.更多还原

【Abstract】 The antitumor activity of binuclear platinum（Ⅱ） complex [{Pt（H₂O）₂I}μ-OOC-COO-{Pt（H₂O）₂I}] was determined by MTT and SRB methods in vitro.At the same time,the anticancer mechanism was studied by flow cytometry,Giemsa and Icp-Ms.The experimental results indicated that the complex had high activity against HL-60、BGC-823,Bel-7402,KB,Hela,HCT-8,MCF-7 and EJ,and the inhibition rate was greater than 70% against HL-60、BGC-823 and Bel-7402 at concentration of 10 μmol·L^-1.The cell cycle was arrested on the G₂+M→G₁,the effect of the complex on apoptosis was not obvious,and the level of total platinum bound to DNA was higher than that of cisplatin under the same conditions.更多还原