【摘要】 目的探讨内皮抑素对喉鳞癌细胞(Hep-2)的作用及其可能的机制。方法①MTT法检测不同浓度(10~50μg/ml)的内皮抑素作用72h和30μg/ml的内皮抑素作用不同时间(24~72h)对Hep-2细胞的影响;②电镜观察Hep-2细胞超微结构的变化;③RT-PCR法检测Hep-2细胞内皮抑素作用组中Survivin基因的表达。结果①MTT检测显示,内皮抑素(20~50μg/ml)能抑制Hep-2细胞的增殖(P<0.05,P<0.01),具有剂量-时间依赖性;②电镜观察,Hep-2细胞内皮抑素作用组均出现凋亡改变;③RT-PCR结果,与正常Hep-2细胞对照组相比较药物作用组Survivin基因表达受到部分抑制,差异呈显著性(P<0.01)。结论内皮抑素能抑制喉鳞癌Hep-2细胞,并具有时间-剂量依赖性,机制可能为诱导细胞凋亡。提示,内皮抑素可能通过诱导喉癌细胞本身的凋亡,对喉癌的生长与转移起到抑制作用。更多还原

【Abstract】 Objective To evaluate the inhibitory effect and the possible mechanism of endostatin on laryngeal carcinoma cells(Hep-2).Methods ①By means of MTT assay,the influences of endostatin with different concentrations(10～50μg/ml) and endostatin(30μg/ml) with different time(24～72h) on the livability of Hep-2 cells were studied;②The changes of Hep-2 cells’ ultrastructures were examined by electron microscope;③After that,Survivin mRNA expression was measured in Hep-2 cells with endostatin(30μg/ml) by RT-PCR test.Results ①The growth of Hep-2 cells was significantly inhibited(P<0.05,P<0.01)by endostatin(20～50μg/ml),what’s more it presents dose-time-dependent;②Apoptosis was observed in the groups of Hep-2 cells with endostatin by electron microscope;③The expression of Survivin in Hep-2 cells was partly inhibited by endostatin in the test of RT-PCR,and it was apparent difference compared with the cells without drugs(P<0.01).Conclusion Endostatin can inhibit the proliferation of Hep-2 cells with time-dose-dependent and it’s mechanism maybe induces the apoptosis.Prompt,Endostatin perhaps inhibit the growth and the transformation of laryngeal squamaous cells carcinoma through inducing the apoptosis of Hep-2 cells.更多还原