【摘要】 目的制备人肝微粒体蛋白多克隆抗体,为进一步研究微粒体蛋白的功能及制备单克隆抗体打下基础。方法用含人肝微粒体高丰度蛋白的蛋白凝胶免疫家兔,再用ELISA和Western blot方法检测血清效价和抗体特异性。结果2只家兔均产生了高效价的抗血清,Western blot结果显示,抗血清可以与相应蛋白结合。结论用含人肝微粒体高丰度蛋白的蛋白凝胶免疫的2只家兔均获得了高效价的抗血清,为深入研究该蛋白的定性、定量及定位奠定了基础,并为将来进行单克隆抗体的制备做前期技术支持。更多还原

【Abstract】 Objective To produce polyclonal antibodies against liver microsome,so that make fundation for research of microsome protein and for producing monoclonal antibodies.Methods Immunizing rabbits with high density gel of microsome protein.Antiserum was obtained and detected by ELISA and Western blot.Results Both of rabbits produced high efficiency antiserum.Western blot showed that the antiserum was able to react with the protein.Conclusion High efficiency antibody of liver microsome is achieved by protein immunization,so make it possible to research the protein and provide feasible technical method for the production of monoclonal antibodies.更多还原