【摘要】 目的研究槲寄生总黄酮苷对缺氧、急性心肌缺血和心律失常的预防作用。方法采用乙醇和有机溶剂萃取方法从槲寄生中提取总黄酮有效成分;用磨口瓶法观察完全密闭小鼠的存活时间;垂体后叶素(1U/kg)致心肌缺血模型,用分光光度法测LDH、MDA、SOD值;用乌头碱(40μg/kg)建立心律失常模型,观测对室颤发生率等的影响。结果耐缺氧实验中给药组小鼠的平均存活时间显著长于对照组(P<0.05);在心肌缺血实验中,给药组血清中LDH、MDA、SOD的含量与模型组有显著差异(P<0.05);在心律失常实验中室性心动过速(VT)、室颤(VF)的发生率有降低趋势,室性早搏出现时间有延长趋势,对窦性节律恢复率没有影响。结论槲寄生总黄酮苷可显著增强小鼠耐缺氧能力;槲寄生总黄酮苷对垂体后叶素引起的大鼠急性心肌缺血损伤具有显著的保护作用;槲寄生总黄酮苷对大鼠心律失常有一定拮抗作用。更多还原

【Abstract】 Objective To detect the protective effect of viscum coloratum total flavonoid(VCTF) on the Hypoxia,myocardial ischemia and arrhythmia.Methods ①Effective component of total flavone was extracted from the VCTF.②Put mice into jars,and recorded the survival time.③Myocardial ischemia model was constructed by injecting hypophysin(1U/kg),and determined serum concentration of LDH,MDA,SOD.④The arrhythmia model was constructed by aconitine(40ug/kg)and observed the occurrence time of VP and so on.Results ①The survival time of VCTF group was longer than that of control group significantly(P<0.05).②In the experiment of myocardial ischemia,serum concentration of LDH,MDA,SOD in treated group were different with the model group obviously(P<0.05).③In the experiments of arrhythmia:the incidence of VT,VF had a decreasing tendency,but the time to occur the ventricular extrasystole had a increasing trend,and there was no influence on the rate of sinus rhythm recovery.Conclusion ①VCTF can increase the hypoxia tolerance of mice.②VCTF has protective effect in myocardial ischemia.③VCTF has antiarrhythmia effect.更多还原