【摘要】 目的观察胃癌组织淋巴管结构和分布特点,探讨微淋巴管密度(LMVD)与肿瘤淋巴转移的相关性。材料和方法临床各期胃癌患者,施行胃癌切除术获得胃癌癌周组织和正常区胃组织标本切片,VEGFR-3抗体免疫组化染色,标记淋巴管,计数组织中LMVD。依据临床病理特征分析LMVD变化。结果胃癌癌周组织与正常区组织石蜡切片各56例,在癌周区处于开放状态的功能性微淋巴管增多。胃癌癌周组织LMVD(22.10±6.99个/HP×200)较远离癌肿区正常胃组织LMVD(10.63±1.90个/HP×200)增多(P=0.000)。胃癌癌周LMVD与淋巴转移、肿瘤大小和分期有关(P<0.05),与分型和是否侵及浆膜无关(P>0.05)。结论胃癌癌周LMVD与肿瘤大小、分期和淋巴转移有密切关系,可以作为肿瘤诱导生成淋巴管研究的量化指标,有助于胃癌的预后判断及治疗方案的评价。更多还原

【Abstract】 Objective:To investigate the relationship between lymphatic microvessel density(LMVD)and clinical pathology of gastric carcinoma.Methods:The expression of VEGFR-3 in 56 gastric cancers(GC)was detected by immunohistochemistry(SABC),by which the LMVD was calculated.Results:The lymphangiogenesis was shown in peritumoral tissue in GCs.The LMVD of peritumoral tissue in GCs(22.10±6.99)was significantly higher than that of normal gastric tissue (10.63±1.90)(P=0.000).LMVD with lymphatic metastasis(25.43±6.33)was higher than that without lymphatic metastasis(16.10±2.93)(P=0.000).LMVD in pTNM Ⅲ+Ⅳ(24.88±6.71)was higher than that in pTNM Ⅰ+Ⅱ(16.22±2.50) (P=0.000).LMVD with big tumor lymphatic metastasis(25.43±6.33)was higher than those without lymphatic metastasis(16.10±2.93)(P=0.000).LMVD in pTNM Ⅲ+Ⅳ(24.88±6.71)was higher than that in pTNM Ⅰ+Ⅱ(16.22±2.50)(P=0.000).LMVD with big tumor (diameter≥5cm)(27.18±6.95)was higher than those with small tumor(diameter<5cm)(19.28±5.33)(P=0.002).There was no significant difference between LMVD and the tumor size and histopathologic type of cancer.(P>0.05).Conclusion:LMVD in GCs showed positive relations with pTNM stage and lymphatic metastasis.更多还原