【摘要】 目的建立芍石护睛胶囊中芍药苷含量的测定方法。方法采用超声提取RP-HPLC法测定,用Hypersil C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-水(27:73)为流动相;检测波长230 nm。结果芍药苷的线性范围为0.074~0.741μg(r=0.9999),平均回收率为100.7%,RSD=2.1%(n=5)。方法精密度的RSD=1.1%(n=5)。结论所建方法分离效果佳、灵敏度高、重复性好,结果准确可靠。更多还原

【Abstract】 OBJECTIVE To establish an RP-HPLC method for determining paeoniflorin in Shaoshi Securing capsule.METHODS The samples were extracted by ultrasound and detected by Hypersil C₁₈ column（250 mm×4.6 mm,5 μm） with methanol-water（27:73） as mobile phase and detection wavelength at UV 230 nm.RESULTS The calibration curve of paeoniflorin was linear in the range of 0.074-0.741 μg（r=0.99995）.The average recovery of sample was 100.7% with the RSD of 2.1%（n=5）.Precision of the analytical method was 1.1%（n=5）.CONCLUSION The method is reproducible and sensitive.更多还原