【摘要】 目的建立开胸顺气滴丸中厚朴酚与和厚朴酚含量的测定方法。方法采用RP-HPLC法,色谱柱为VP C18(150 mm×4.6 mm,5μm);流动相为甲醇-乙腈-水(50:20:40);流速1.0 m l.m in-1;柱温25℃;检测波长294 nm。结果厚朴酚的线性范围为0.2961~2.9610μg(r=0.9996);和厚朴酚的线性范围为0.1608~1.6080μg(r=0.9996)。厚朴酚的平均回收率为99.17%±1.66%,RSD=1.3%(n=6);和厚朴酚的平均回收率为99.04%±2.06%,RSD=1.7%(n=6)。结论所用方法简便、准确,可用于开胸顺气滴丸中厚朴酚与和厚朴酚的含量测定。更多还原

【Abstract】 OBJECTIVE To establish a method for determination of magnolol and isomagnololin in Kaixiong Shunqi drops.METHODS An RP-HPLC method was adopted.The analysis was carried out on an analytical column C₁₈（150 mm×4.6 mm,5 μm）.The mobile phase consisted of methanol-acetonitrile-water（50:20:40） and the flow rate was 1.0 ml·min^-1.The detective wavelength was set at 294 nm with column temperature of 25℃.RESULTS The lienar range was 0.2961-2.9610 μg（r=0.9996） and the average recovery was 99.17%±1.66% with the RSD of 1.3%（n=6） for magnolol,respectively.The linear range was 0.1608-1.6080 μg（r=0.9996）and the average recovery was 99.04%±2.06% with the RSD of 1.7%（n=6）for isomagnololin,respectively.CONCLUSION The method is simple,reproducible and suitable for determination of magnolol and isomagnololin in Kaixiong Shunqi drops.更多还原