【摘要】 目的建立测定悬浮培养黄芩细胞中黄芩苷含量的方法。方法采用HPLC法,色谱柱为Agilent Zorba×80 Extend-C18柱(150 mm×4.6 mm,3.5μm),流动相为甲醇-0.5%磷酸(50:50),柱温30℃,流速1.0 m l.m in-1,检测波长280 nm。结果线性范围为0.244~2.440μg(r=0.9996),平均回收率为99.17%,RSD=1.39%(n=6)。结论所用方法简便、准确、重复性好,可作为规模化生产黄芩药材细胞质量控制的有效方法。更多还原

【Abstract】 OBJECTIVE To determine the baicalin content in suspension culture of Scutellaria baicalensis Georgi cells.METHODS An HPLC method was adopted.The separation was performed on Zorba×80 Extend C₁₈ column（150 mm×4.6 mm,3.5 μm） with a mobile phase of methanol-0.5% phosphoric acid（50:50）.The flow rate was 1.0 ml·min^-1 and the detective wavelength was 280 nm.RESULTS The linearity of the method was well within the range of 0.244 to 2.440 μg（r=0.9996） and the average recovery of baicalin was 99.17% with RSD of 1.39%（n=6）.CONCLUSION The method is simple,accurate and reliable.It can be used for quality control of Suctrllaria baicalensis cell production in factory in future.更多还原