【摘要】 目的优选射干的提取纯化工艺。方法运用正交试验,筛选最佳提取工艺;用大孔吸附树脂对提取液进行纯化;用HPLC法测定次野鸢尾黄素的含量并评价工艺。结果提取工艺以8倍量50%乙醇,提取3次,每次1 h最佳;纯化工艺以进样量为5BV(树脂床体积),用8BV的70%乙醇进行洗脱效果最佳。经纯化后射干次野鸢尾黄素可达40.9 mg.g-1。结论该法简单可行,分离效果好,适于工业化大生产。更多还原

【Abstract】 OBJECTIVE To explore the optimum extraction and purification conditions of Belamcanda chinensis（L.）DC.METHODS The orthogonal design was adopted to optimize the alcohol extraction process.The macroporous resin column was used for the purification and the contents of irisforentin were measured as markers to evaluate the effect of the extraction and purification of different processes by RP-HPLC.RESULTS The final extraction process was adding 8 times amount of 50% alcohol and extracting 3 times for 60 min each time.The purification process was the best adsorptive capacity of 5BV and the volume of 70%ethanol as eluant 8BV.Finally,the content of irisforentin could reach to 40.9 mg·g^-1.CONCLUSION This method is simple and feasible,and suitable for industrial requirements.更多还原