【摘要】 目的建立测定人尿液中甲磺酸帕珠沙星浓度的方法。方法采用HPLC法,取待测尿样加入内标适量,用蒸馏水稀释后离心,取上清液进样测定。结果将帕珠沙星峰面积和内标诺氟沙星峰面积比值对浓度进行回归,得回归方程。线性范围为10 n.gm l-1~50μg.m l-1(r=0.9992,n=10)。相对回收率98.08%~102.70%,日内、日间RSD分别小于1.5%和3.5%。结论所建方法准确可靠,操作简便,适用于尿药浓度的测定。更多还原

【Abstract】 OBJECTIVE To establish an analytical method for determination of Pazufloxacin mesylate in human urine.METHODS HPLC method was adopted to detemine Pazufloxacin mesylate in human urine.RESULTS The calibration curve was linear within the range of 10 ng·ml^-1-50 μg·ml^-1（r=0.9992,n=10）,the relative recoveries were from 98.08% to 102.70%.The RSD in a day and between days were lower than 1.5% and 3.5%,respectively.CONCLUSION RP-HPLC method is simple,economic,sensitive and accurate.It is suitable for determination of Pazufloxacin mesylate levels in human urine.更多还原