【摘要】 目的建立测定水解后的川木通药材中齐墩果酸含量的方法。方法采用HPLC法。Alltima C18色谱柱(250 mm×4.6 mm,5μm);流动相为甲醇-水-冰醋酸-三乙胺(89∶11∶0.04∶0.02);检测波长207 nm;流速1.0 ml.min-1;柱温45℃。结果齐墩果酸色谱峰分离度良好,线性范围为0.1003~8.024μg(r=0.9999,n=5),方法的检测限和定量限分别为0.3μg.ml-1和1.0μg.ml-1。平均回收率为96.1%,RSD=2.8%(n=5)。结论所用结果准确,重复性好,专属性强,可为川木通药材质量控制提供科学的依据。更多还原

【Abstract】 OBJECTIVE To develop an HPLC method for the content determination of oleanolic acid in Caulis clematidis armandii after hydrolysis.METHODS In the system of HPLC,a Alltima C₁₈（250 mm×4.6 mm,5 μm） column was used with a mixture of methanol-water-acetic acid-triethylamine（89∶11∶0.04∶0.02） as the mobile phase.The measuring wavelength was set at 207 nm.The flow rate was 1.0 ml·min^-1 and the column temperature maintained at 45 ℃.RESULTS The oleanolic acid peak in chromatograms was completely separated from other peaks.The linear range of oleanolic acid was 0.1003-8.024 μg（r=0.9999,n=5）.The detection limit and quantitation limit of this method were 0.3 μg·ml^-1 and 1.0 μg·ml^-1,respectively.The average recovery of oleanolic acid was 96.1%（RSD=2.8%,n=5）.CONCLUSION The method is accurate,precise and reproducible.It can be used to evaluate the quality of Caulis clematidis armandii.更多还原