【摘要】 目的探讨自噬基因Beclin1在宫颈鳞癌中的蛋白表达以及与肿瘤发生发展的关系。方法采用免疫组化SP法检测81例宫颈鳞癌、20例宫颈上皮内瘤样病变(CIN,~级)和20例正常宫颈组织中自噬基因Be-clin1的蛋白表达。结果宫颈鳞癌组织中Beclin1阴性、弱阳性、强阳性表达率依次是43.2%(35/81)、34.6%(28/81)、22.2%(18/81),与正常宫颈和宫颈上皮内瘤样病变组相比,Beclin1在宫颈鳞癌中的表达显著下调(P=0.011)。宫颈鳞癌中Beclin1的表达在不同FIGO分期、年龄、宫颈肌层浸润深度、宫颈肿瘤大小、大体类型间的差异无统计学意义。但在有无盆腔淋巴结转移、不同肿瘤分化程度间差异有统计学意义(P<0.05)。结论自噬基因Beclin1在宫颈鳞癌中蛋白表达下调,并与宫颈鳞癌盆腔淋巴结转移和肿瘤分化程度有关。更多还原

【Abstract】 Objective Autophagy gene Beclin 1 plays an important role in several types of human cancers.So we, in this study, employed the immunohistochemical manner to detect the Beclin 1 gene expression and explore its clinical significance in cervical aquamous cell carcinoma. Metheds SP immunohistochemistry technique was used to detect the expression of Beclin 1 gene in specimens of 81 cervical squamous cell carcinoma, 20 cervical intraepithelial neoplasm (CIN, Ⅱ-Ⅲ), and 20 normal cervix. Correlations between the expressions of Beclin 1 gene and the clinicopathologic factors of cervical squamous cell carcinoma were statistically analyzed. Results The rates of negative, weak, strong expression of Beclin 1 in cervical squamous cell carcinoma were 43.2% (35/81), 34.6% (28/81) or 22.2% (18/81) respectively, and the significantly lower than those in normal cervix and CIN Ⅱ-Ⅲ(P=0.011).The Beclin 1 expressions did not associate with FIGO stage, age, depth of cervical infiltration, tumor size, and gross type of cervical lesion (P<0.05), but were related to pelvic lymph node metastases and histological tumor grade (P<0.05). Conclusions Expression of autophagy gene Beclin 1 decreases in cervical aquamous cell carcinoma, and is closely related to pelvic lymph node metastases and histological tumor grade.更多还原