【摘要】 目的观察与小鼠乳腺肿瘤病毒(mousemammarytumorvirus,MMTV)相似的基因序列在131例中国女性乳腺癌中的表达。方法取131例中国女性乳腺癌组织标本,提取组织DNA,进行巢式PCR,检测癌组织中与MMTV相似的基因序列的表达情况,结合病理结果进行分析,并将所得结果与已知各国的阳性率相比较。结果131例标本中22例表达阳性,阳性率为16.8%。阳性表达率与病理分型分期不相关。已报道的美国(38.5%)、意大利(37.7%)、阿根廷(31%)、澳大利亚(42.2%)、越南(0.8%)和奥地利(0%)人群阳性率与中国(16.8%)的阳性率相比较总体上差异存在统计学意义(P<0.001)。美国、意大利、阿根廷、澳大利亚和奥地利与中国和越南的阳性率比较差异存在统计学意义(P<0.001)。中国分别与美国、意大利、阿根廷、澳大利亚、越南和奥地利之间的阳性率相比差异存在统计学意义(P值均<0.05)。结论与MMTV相似的基因序列在中国乳腺癌组织中有表达,提示MMTV的人类同源病毒在癌组织中的存在可能;美国、意大利、阿根廷、澳大利亚、越南、奥地利和中国人群中已报道的序列阳性率总体上存在差异,与乳腺癌的流行病学存在部分一致性,符合地域差异特点。更多还原

【Abstract】 Objective To analyse the expression of mouse mammary tumor virus-like (MMTV-like) gene sequencesin breast cancer of Chinese women. Methods Using nested PCR that amplify MMTV-like gene sequences, we tested the breast cancer tissues from 131 Chinese patients. The result was analyzed and compared with postoperative pathology and prevalence that had been reported from different populations. Results MMTV-like gene sequences were amplified in 22 of 131 (16.8%) breast cancer biopsy tissues from Chinese patients, which had no relation to pathology, but had the significant difference from other populations. Conclusion MMTV-like gene sequences can be found in breast cancer tissue from Chinese women with breast cancer. This supports the contention that these sequences may represent a virus associated with human breast carcinogenesis. The differences in prevalence of MMTV-like gene sequences and breast cancer rates among populations may be related to geographic distribution.更多还原