【摘要】 目的检测不同FIMA基因型牙龈卟啉单胞菌在我国慢性牙周炎患者中的分布状况,及其与牙周炎的相关性。方法收集101例慢性牙周炎患者的龈下菌斑,采用P.GINGIVALIS16S RRNA和5种FIMA基因型的特异引物,用PCR法检测不同FIMA基因型P.GINGIVALIS的分布;记录取样牙六个位点(近颊、颊正中、远颊、近舌、舌正中、远舌)的牙周临床指标,包括牙周探诊深度,临床附着丧失和探诊出血。结果16S RRNA PCR,P.GINGIVALIS检出率为88.1%。大多数受检牙龈下菌斑只检测出一种FIMA基因型(65.1%),FIMA的总检出率最高为43.8%,其次为FIMA,检出率为40.4%;FIMA型和FIMA型P.GINGIVALIS在轻中度和重度牙周组的检出率均最高。结论FIMA型和FIMA型P.GINGIVALIS是我国牙周炎患者龈下菌斑中的优势菌株,表明FIMA型和FIMA型P.GINGIVALIS与我国人群牙周炎的发生发展密切相关。更多还原

【Abstract】 Objective To investigate the distribution of fimA genotype of Porphyromonas gingivalis in Chinese periodontitis patients and try to find the relationship between fimA genotype and chronic periodontitis. Methods Subgingival plaque samples were collected from 101 periodontitis patients. P.gingivalis 16S rRNA primer and fimA type-specific primer were designed. The distribution of fimA genotype in periodontitis patients was detected by PCR. Clinical periodontal indices (PPD, CAL and BOP) were measured at the sample tooth’s six points; namely, the mesio-,mid-,distobuccal and mesio-,mid-,distolingual points. Results P.gingivalis was detected in 89 periodontitis patients (88.1%). Among them, a single fimA genotype was detected in most subgingival plaque samples (65.1%), and the most prevalent fimA genotype was type Ⅱ (43.8%), followed by type Ⅳ (40.4%); Type Ⅱ fimA and Ⅳ fimA were more frequently detected in mild/moderate periodontitis group and severe periodontitis group. Conclusion The findings indicate that P.gingivalis with type Ⅱ fimA and Ⅳ fimA are more predominant in Chinese periodontitis, and the organisms are involved in the destructive progression of periodontitis in Chinese.更多还原