【Abstract】 A reversed phase high performance liquid chromatographic method was described for the determination of trace formaldehyde in beer with precolumn derivatization and UV detection.Formaldehyde in beer was directly derived with acetylacetone reagent to yield 2,6-diethyl-3,5-diacetyl-1,4-dihydropyridin(DDL).DDL was extracted by the reaction solution passing through a Waters O ASIS HKLB cartridge and then was analyzed by isocratic HPLC on a Xterra RR18 column(150x3.9mm I.D.5μm)with acetonitrile-water containing 0.075% TFA(1/7,v/v)as mobile phase,detection at 254nm and column temperature at 40℃.The linearity of the method is satisfactory in the range of 0.09935~9.935mg/L with the correlation coefficient of 0.9999.The recoveries range within 96.1~102.8%.The relative standard deviation is less than 4.2% and the limit of detection is 0.4ng.更多还原