【摘要】 [目的]查明2004年夏秋季福州市部分地区发生疑似登革热暴发流行的病原。[方法]采用C6/36细胞对患者血清标本进行病毒分离,应用免疫荧光、RT-PCR方法进行鉴定。[结果]从27份患者血清标本中分离出16株病毒,经鉴定为登革Ⅰ型病毒。[结论]福州市本次暴发流行由登革Ⅰ型病毒所致,系福建省首次从暴发流行患者血清中分离出登革Ⅰ型病毒,为登革热流行的防治提供科学依据,同时对进一步研究其流行特点和诊断试剂奠定了基础。更多还原

【Abstract】 [Objective] To study the etiological agent of dengue fever outbreak in Fuzhou in 2004.[Methods] The virus in patient’s serum specimens was isolated by using C6/36 cell.The isolates were identified with an indirect immunofluorescence test and RT-PCR method.[Results] Totally 16 strains of dengue virus were isolated from 27 specimens and identified as typeⅠ.[Conclusion] The outbreak in Fuzhou in 2004 was caused by dengue virus typeⅠ.This study result will be useful for the epidemiological research and producing of diagnostic kit of dengue fever in the future.更多还原