【摘要】 [目的]分析1991—2004年广东省乙型流感病毒抗原变异和流行情况。[方法]用9~11日龄鸡胚和(或)MDCK细胞分离流感病毒,病毒鉴定用常量红细胞凝集抑制法(HI);人血清流感抗体检测用微量半加敏红细胞凝集抑制法。[结果]广东省一直有乙型流感流行,并从人群中分离到流感病毒2 916株,乙型流感病毒占23.3%。乙型流感病毒两大谱系交替为优势株,除1994、1997及2002年以Victoria系为主外,其余各年以Yamagata系为主。乙型流感病毒抗原性不断发生漂移,历年一般人群血清乙型流感抗体检测阳性率在28.3%~73.9%。[结论]1991—2004年广东省乙型流感病毒存在差异较大的两个谱系,病毒谱系的轮换和抗原漂移导致乙型流感不断流行。更多还原

【Abstract】 To analyze the activity status,antigenic drift and epidemiological characteristics of influenza B in Guangdong,China for the disease control(1991-2004).Influenzas B viruses were isolated or recovered by embryonated eggs aged 9-11 days and/or MDCK cells,and the viruses were identified by hemagglutination inhibition(HI) method.The serum antibody was detected by micro HI.There were influenza B virus epidemic in Guangdong during these years.2 916 strains were isolated and 23.3% of them were influenza B.Two predominant lineages of influenza B virus co-circulated in Guangdong.The main lineage was Yamagata,but Victoria was more active in 1994,1997 and 2002.Antigenic drift occurred there continuously.The positive rate of anti-B antibody were ranged from 28.3% to 73.9% in general population.During 1991-2004,two major lineages of influenza B circulated in Guangdong.The alternation of two lineages and antigenic drift continuously resulted in influenza B epidemic.更多还原