【摘要】 在碱性介质中,CO32-对H2O2氧化鲁米诺化学发光反应具有重要作用,荧光素钠对该反应具有很强的增敏作用.据此,建立了化学发光法测定二氧化碳的新方法.方法的线性范围为1.0×10-10～5.0×10-6mol?L-1CO32-,检出限为1.2×10-11mol?L-1CO32-(相当于5.3×10-10g?L-1CO2).该方法用于室内外空气中二氧化碳含量的测定,相对标准偏差1.8%～2.1%(n=11),加标实验回收率97.6%～101.4%.论文还探讨了反应的发光机理,发光反应很可能是由溶液中的CO32-与H2O2作用而产生的活性自由基引发,荧光素钠对发光的增敏作用为化学能量转移过程.更多还原

【Abstract】 The chemiluminescent (CL) reaction for the oxidation of luminol with hydrogen peroxide in an alkaline medium was greatly affected by the presence of carbonate. The intensity of CL reaction was evi- dently enhanced by the addition of uranine. Based on this, a novel CL method for the determination of car- bon dioxide was established. The method provided the detection a wide linear concentration rang from 1.0× 10-10 to 5.0×10-6 mol?L-1 and a low detection limit of 1.2×10-11 mol?L-1 carbonate or 5.3×10-10 g?L-1 carbon dioxide. It has been applied to determination of carbon dioxide in indoor and outdoor air with relative standard deviations (RSD) of 1.8%～2.1% (n=11). The recoveries of the spiked samples were 97.6%～ 101.4%. A reasonable mechanism was also proposed. The CL reaction is probably triggered by the interac- tion of H2O2-K2CO3 and then enhanced by uranine via chemical energy transfer from luminol to uranine.更多还原