【摘要】 醛缩酶C是生命代谢过程中重要糖酵解同工酶之一.应用二维凝胶电泳分离卵巢癌细胞中高表达的果糖二磷酸醛缩酶C,并通过基质辅助激光解吸电离飞行时间串联质谱对其进行分析与鉴定,结果表明应用高分辨二维凝胶电泳分离,结合生物质谱联用技术分析和鉴定未知蛋白具有简单快捷的特点.更多还原

【Abstract】 Fructose-bisphosphate aldolase C is one of important glycolytic isozymes in the metabolic proc- ess. The fructose-bisphosphate aldolase C which highly expressed in the human ovarian adenocarcinoma cell has been separated by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF MS). The results indicate that MALDI-TOF/TOF MS in combination with the high resolution two dimensional gel electrophoresis is a simple and fast method for identification of unknown proteins.更多还原