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含氨基萘酰亚胺类DNA嵌入剂切割质粒DNA
Plasmid DNA Cleavage by Aminoalkylnaphthalimide-intercalator Containing Amino Groups
【摘要】 合成了一系列含氨基的萘酰亚胺类化合物以开发新型的DNA切割剂。琼脂糖电泳分析表明,100μmol/L含氨基萘酰亚胺化合物在70℃、pH7·5的条件下能有效切割超螺旋质粒DNA,而且切割产物均为接近原质粒大小一半的DNA线性片段。
【Abstract】 A series of aminoalkylnaphthalimides were synthesized in order to develop novel DNA cleavers. Agarose gel electrophoresis showed supercoiled plasmid DNA with various sizes could be efficiently cleaved in the presence of 100 μmol/L of aminoalkylnaphthalimides at 70℃, pH 7.5. Interestingly, all of cleaved products are close to half-sized DNA fragments in a linear form.
【关键词】 萘酰亚胺;
质粒DNA;
DNA嵌入剂;
人工核酸酶;
【Key words】 Naphthalimide; Plasmid DNA; DNA Intercalator; Artificial Nuclease;
【Key words】 Naphthalimide; Plasmid DNA; DNA Intercalator; Artificial Nuclease;
【基金】 国家重点基础研究计划(2003CB114400);国家自然科学基金(20536010,20576016)资助项目
- 【文献出处】 化学通报 ,Chemistry , 编辑部邮箱 ,2006年10期
- 【分类号】Q78
- 【被引频次】7
- 【下载频次】374