【摘要】 合成了一系列含氨基的萘酰亚胺类化合物以开发新型的DNA切割剂。琼脂糖电泳分析表明,100μmol/L含氨基萘酰亚胺化合物在70℃、pH7·5的条件下能有效切割超螺旋质粒DNA,而且切割产物均为接近原质粒大小一半的DNA线性片段。更多还原

【Abstract】 A series of aminoalkylnaphthalimides were synthesized in order to develop novel DNA cleavers. Agarose gel electrophoresis showed supercoiled plasmid DNA with various sizes could be efficiently cleaved in the presence of 100 μmol/L of aminoalkylnaphthalimides at 70℃, pH 7.5. Interestingly, all of cleaved products are close to half-sized DNA fragments in a linear form.更多还原