【摘要】 目的采用MBECTM-Device研究防龋中药五倍子多酚性化合物(GCE)、五倍子B(GCE-B)、蜂房95%组分(NVE1)对多菌种生物膜细菌的生长抑制作用,建立防龋中药药效学的药物敏感实验方法,为临床实验提供客观的药物作用的浓度范围。方法选择与龋病发生密切相关的4种口腔细菌形成多菌种生物膜,药物为GCE、GCE-B、NVE1,扫描电镜观察口腔细菌在MBECTM-Device上形成细菌生物膜的能力和形成情况;采用MBECTM-HTP-Assay测定药物对口腔细菌的最小抑菌浓度(MIC)和对细菌生物膜的最小生物膜清除浓度(MBEC)。结果在MBECTM-Device提供的桩钉表面上口腔细菌能形成良好的生物膜结构,从不同时段取出的样本可以观察到细菌从定植粘附到生物膜形成以及生物膜成熟结构。GCE、GCE-B、NVE1对口腔细菌均有良好的抑制作用。GCE、GCE-B对口腔生物膜细菌有很好的抑制作用,但是NVE1对口腔生物膜细菌的抑制作用较差。药物对口腔生物膜细菌的MBEC是MIC的2～16倍。结论中药五倍子多酚性化合物和五倍子B对口腔生物膜细菌有很好的抑制和清除效应,MBEC比MIC能更客观地反映药物作用的浓度范围。更多还原

【Abstract】 Objective Using MBECTM-Assay to assay minimal biofilm eradication concentration(MBEC)of Galla Chinensis and Nidus Vespae to oral bacterial biofilm.To set up traditional Chinese medicine susceptibility pharmacodynamic empirical study methods of oral bacterial biofilm.Methods Cariogenic bacteria strains were selected(Streptococcus mutans ATCC 25175,Streptococcus sanguis ATCC 10556,Lactobacillus rhamnosus AC 413,Actinomyces naeslundii WVU 627)in this study.Extraction components of Galla Chinensis were GCE(aqueous extract),GCE-B(30% alcohol extract)and extraction components of Nidus Vespae were NVE1(95% alcohol extract).①To observe oral bacterial biofilm formatiom in MBECTM-Device at different time.②MBECTM-HTP-Assay:The minimal inhibitory concentration(MIC)and minimal biofilm eradication concentration(MBEC)of GCE,GCE-B and NVE1 to oral bacteria strains were determined.Results Oral bacterial biofilm were readily formed on the lid of MBECTM-Device under selected condition(observed by SEM).Oral cariogenic bacteria growing as plankton populations were sensitive to GCE,GCE-B and NVE1.To GCE,GCE-B and NVE1,oral cariogenic bacterial biofilm were 2-16 times less susceptible than growing plankton bacteria.GCE and GCE-B were the most effective medicine against oral cariogenic bacterial biofilm.NVE1 were effective in killing oral-bacterial biofilm at relatively high concentration.Conclusion GCE and GCE-B were effective medicine against oral cariogenic bacterial biofilm.MBEC(minimal biofilm eradication concentration)can provide a relative accurate medicine concentration for clinical test.更多还原