【摘要】 目的探讨正畸牙受力后龈沟液内白细胞介素-1(βIL-1)β和肿瘤坏死因子-(αTNF-)α水平的动态变化及其生物学意义。方法选择15例正畸患者为研究对象,将每位患者的4颗尖牙按左右侧随机分入实验组和对照组。实验组用橡皮链加力拉尖牙向远中,对照组尖牙不加力。分别在戴矫治器前,戴矫治器后1、24、48、72和168h时收集两组尖牙远中颊面龈沟液,应用放射免疫法测定龈沟液内IL-1β和TNF-α的含量。结果实验组在加力24h后IL-1β和TNF-α水平开始升高,72h达到最高,168h基本恢复至基线水平;对照组在整个实验过程中IL-1β和TNF-α含量均保持在基线水平。48h和72h实验组IL-1β和TNF-α含量与对照组相比有显著性差异(P<0.05)。24h实验组TNF-α含量与对照组有显著性差异(P<0.05),而IL-1β含量与对照组相比无显著性差异(P>0.05)。结论牙齿正畸过程中受机械力的作用龈沟液内IL-1β和TNF-α水平发生动态变化,表明这两种生物活性因子早期即参与牙齿移动和牙槽骨重建过程。更多还原

【Abstract】 Objective To investigate the dynamic changes of interlukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α) in gingival crevicular fluid(GCF) during orthodontic tooth movement,and to discuss the biological significance.Methods Fifteen patients were chosen as subjects.For each patient,upper and lower canines at one side having one treatment for distal movement by elastic chain served as the experimental teeth,whereas the contralateral ones were used as controls.The GCF were taken before activation and at 1,24,48,72,168 hours respectively after initiation of the experiment.The levels of IL-1β and TNF-α in GCF were determined by radioimmunoassay.Results The levels of IL-1β and TNF-α in experimental group began to increase at 24 hours and reached to its peak value at 72 hours after initiation of the experiment,but their levels returned to baseline at 168 hours.Both of them,however,remained at the baseline level in control group.The changes of the two cytokines level were found statistically significant at 48 and 72 hours(P<0.05) between experimental and control group.No statistically significant were observed before activation and at 1,168 hours after application of orthodontic forces(P>0.05) between experimental and control group.Conclusion The levels of IL-1β and TNF-α in gingival crevicular fluid experience dynamic changes during the early phase of orthodontic treatment,indicate that they might play an important role in the process of alveolar regeneration and tooth movement.更多还原