【摘要】 目的探讨甲状旁腺相关蛋白(PTHrP)对鼠胚髁突软骨细胞增殖和分化的影响。方法在体外分离培养鼠胚髁突软骨细胞的基础上,观察PTHrP对其软骨结节形成、碱性磷酸酶(ALP)活性等的影响。结果研究发现加入浓度分别为0.01nmol/L、0.1nmol/L、1nmol/L、10nmol/L的PTHrP后,0.01nmol/L组与对照组相比,形成的软骨结节数量在统计学上无显著性差异(P>0.05),而软骨结节数量从0.1nmol/L组开始与对照组相比明显增加,统计学上有显著性差异(P<0.05)。而ALP活性在0.1—10nmol/L组与对照组相比明显升高,统计学上有显著性差异(P<0.05)。阻断其受体后,实验组和对照组软骨结节形成的数目明显减少(P<0.05),而对其ALP活性的影响在实验组和对照组之间无显著性差异(P>0.05)。结论PTHrP通过其受体具有促进髁突软骨细胞增殖和分化的作用,其调控机制与其在生长板软骨细胞及下颌膜内成骨中的作用相似。更多还原

【Abstract】 Objective To investigate the regulatory mechanism of parathyroid hormone-related protein(PTHrP) in proliferation and differentiation of chondrocytes of condyle in fetal mouse.Methods Chondrocytes of condyle in fetal mouse were separated and cultured in vitro,the influence of PTHrP on proliferation and differentiation was observed.Results After two weeks′ culture in 0.01 nmol/L,0.1 nmol/L,1 nmol/L,10 nmol/L human PTHrP,there was significant difference in the number of cartilage nodule formed between experiment group and control group(P<0.05),and there was no significant difference in 0.01 nmol/L group(P>0.05).Alkaline phosphatase(ALP) activity was significantly intensified in experiment group and control group(P<0.05).Meanwhile,it was found that this function of promotion was lessened after anti-PTHR antibody used.Conclusion It can be seen that PTHrP,via its receptor,can promote proliferation and differentiation of chondrocytes of condyle,which resemble its modulation mechanism in epiphyseal growth plate cartilage intramembrane in mandibule.更多还原