【摘要】 目的采用重建基底膜细胞侵袭实验,研究直接分离自舌癌组织旁的癌相关成纤维细胞(CAFs)对舌癌细胞株侵袭特性的影响,探讨CAFs在口腔鳞癌发展中的作用。方法以Matrigel模拟重建基底膜,采用Transwell小室建立口腔CAFs与舌癌细胞株Tca8113的交互作用模型,观测CAFs对Tca8113侵袭特性的影响。结果与正常成纤维细胞(NFs)相比,CAFs能促使更多的Tca8113细胞穿透Matrigel(P<0．05)。结论口腔CAFs能促进舌癌细胞株Tca8113 的侵袭,在口腔鳞癌发展中起重要作用。更多还原

【Abstract】 Objective To observe the effect of oral carcinoma-associated fibroblasts (CAFs) on the invasion of a lingual carcinoma cell line, and to elucidate the role of CAFs in oral squamous cell carcinoma (OSCC) progression. Methods Matrigel was used to remodel the basement memberane, and the interaction model between primary oral CAFs and lingual carcinoma cell line Tca8113 was established by Transwell chamber to observe the effect of CAFs on the invasion of Tca8113. Results Compared with normal fibroblasts (NFs), CAFs promoted more Tca8113 cells to penetrate Matrigel (P<0.05). Conclusion Oral CAFs can promote the invasion of lingual carcinoma cell line Tca8113, and play a key role in OSCC progression.更多还原