【摘要】 利用简单节杆菌对6α-甲基可的松C1,2位脱氢,考察了脱氢反应温度、pH值以及底物的投料浓度和方式对底物转化率的影响,得到了较好的反应条件为脱氢反应温度33℃,pH值7.0~7.2,拟结晶投料方式增加底物的投料浓度。在3.7 L发酵罐中进行脱氢反应初步放大试验,得到了菌体生长及底物转化率曲线。利用遗传算法对培养基组成进行了优化,在摇瓶培养中当投料浓度为1.0%时,底物的转化率提高到94.51%。更多还原

【Abstract】 Arthrobacter simplex was applied in the C_1,2 dehydrogenation of 6α-methylcortisone to produce 6α-methylprednisolone.Experimental parameters of this biotransformation including（dehydrogenation） temperature,initial substrate pH and concentration of 6α-methylcortisone were（investigated） in detail and optimum reaction conditions（dehydrogenation temperature 33 ℃,initial substrate pH（7.0-7.2）,（6α-methylcortisone） concentration 1.0%）were obtained.Furthermore,the scale up of（dehydrogenation） was carried out in a 3.7 L fermentor and the course of arthrobacter（simplex） growth and（6α-methylcortisone） conversion were obtained.Finally,the optimization method of genetic（algorithm） was used to optimize ingredient of substrate,and 94.51% of 6α-methylcortisone conversion was（obtained） under the optimized conditions when the initial 6α-methylcortisone（concentration） was（1.0%） in shaking flasks.更多还原