【摘要】 采用荧光分光光度法测定游离氨基酸。在pH=6.0的乙酸-乙酸钠缓冲溶液中,氨基酸与乙酰丙酮-甲醛体系反应,产生黄绿色荧光,试验了体系酸度、试剂加入次序及用量、反应温度、反应时间对体系荧光强度的影响。以丙氨酸为例进行试验,丙氨酸浓度与体系荧光强度在1.0~20.0μg/mL范围内有良好的线性关系,检出限为0.05μg/mL。更多还原

【Abstract】 The free amino acid was determined by fluorescence spectrophometry.In HAc-NaAc buffer solution with pH of 6.0,the amino acid reacts with acetylace-formaldehyde system to from the resultant which sends yellow green fluorescence.The effect of different acidity,reagent order,reagent amont,thermal temperature and heating time on fluorescent intensity were studied.Taking the alanine for example,the alanine concentration was linear with the fluorescence intensity in the range of 1.0-20.0 g/mL,the detection limit was 0.05 g/mL.更多还原