【摘要】 提出了一种聚丙烯氯化铵(PAH)-纳米金固定抗体的压电免疫传感器界面的构建方法。先在压电石英晶振的金电极表面自组装一层半胱氨酸单层膜,通过戊二醛交联带大量NH2基的聚电解质PAH,随后在PAH膜表面自组装一层纳米金粒子,以静电吸附作用固定IgG抗体,研制成一种新的压电免疫传感器的界面,用于对相应抗原的检测。研究了PAH浓度及抗体固定化等实验条件的影响,探讨了传感器的主要响应特性与再生性能,并与戊二醛直接固定的传感器的性能进行了比较。结果表明,前者固定的抗体的活性较高,响应频率较大,检测的线性范围较宽,非特异性吸附小,能有效地改善传感器的灵敏度和检测限,而且容易进行传感器的再生。更多还原

【Abstract】 In this paper, polyelectrolytes and the self-assembled technology are combined for a novel biosensing interfacial design applied to an immunosensor for detecting human IgG. The QCM immunosensor as a model transducer was fabricated with poly(allylamine hydrochloride) (PAH) and then with gold nanoparticles resulting in a PAH-gold nanoparticles adsorption procedure for goat-anti-human IgG. The conditions of immobilizing goat-anti-human IgG are optimized in detail. And the results revealed that the proposed procedure was superior to the traditional glutaraldehyde immobilization method. The proposed immunosensor in optimal conditions has a linear detection range of 5～550 μg/mL for human IgG detection. Sensors obtained according to the new immobilization procedure exhibit better immunosensing performances including high sensitivity, nonspecific adsorption and favorable operational stability etc. Moreover, the proposed immunosensor has advantage in the simple and advantageous regeneration process. Analytical results indicate that the developed immobilization procedure is a promising alternative for the immobilization of biorecognition element on the electrode surface.更多还原