【摘要】 目的:建立山药中尿囊素含量的HPLC的测定方法,考察各地山药质量的优劣。方法:采用ODS-C18(200mm×4.6mm,dp5μm);流动相:甲醇-水(1∶9);流速:0.5ml/min;柱温:30℃;检测波长:224nm。结果:尿囊素在0.05~0.5μg范围内线性关系良好,r=0.9998;尿囊素的平均回收率为101.8%,RSD=2.0%(n=5)。结论:该方法可为山药质量控制提供可靠手段,各产地山药中尿囊素含量差异较大,其中以“怀山药”中尿囊素的含量最高。更多还原

【Abstract】 Objective: To establish a HPLC method for the determination of allantion in Rhizoma Dioscoreae from different producing areas. Methods: Separation and determination of allantion was achieved by using an Betasil C18 colunm and a mobile phase of methanol-water(1∶9,V/V).The flow rate was 0.5 ml/min, temperature at 30℃and detection wavelength at 224 nm. Results: A good linearity of allantoin was in the range of 0.05～0.5 μg, r=0.9998; the average recovery rate was 101.8%,RSD=2.0(n=5). Conclusion: Advantages of improved retention and separation of allantion, and less complicated sample preparation exit over current methods. Rhizoma Dioscoreae from Henna Provine is of the best quality by the Content of allantion in Rhizoma Dioscoreae.更多还原