【摘要】 目的:探讨在不同温度下过滤去除白细胞时对红细胞溶血的影响,寻找防止其溶血的措施。方法:将保存5d的红细胞悬液40 u随机分为对照组和试验组各20 u,前组红细胞悬液于4℃储血冰箱中取出后立即进行过滤,后组红细胞悬液置于35℃水浴箱复温10 m in再行过滤。测定各组红细胞悬液过滤前后的血浆K+和游离血红蛋白浓度。同时对保存5 d的650 u红细胞悬液(包括4℃立即过滤220 u红细胞悬液和经35℃复温10 m in后过滤430u红细胞悬液)滤后血浆的目测溶血情况进行回顾性分析。结果:对照组红细胞悬液滤后血浆K+、游离血红蛋白浓度分别为(6.41±0.16)mm o l/L、(37.84±6.15)m g/L,比滤前(5.62±0.15)mm o l/L、(23.31±4.24)m g/L明显升高(P<0.05);试验组滤后K+、游离血红蛋白浓度(5.72±0.16 mm o l/L、27.93±5.28 m g/L)与滤前(5.63±0.15 mm o l/L、23.51±4.26 m g/L)比较无显著性差异(P>0.05),但比对照组滤后明显降低(P<0.05)。两组滤前K+、游离血红蛋白浓度均无显著性差异(P>0.05)。4℃立即过滤与经35℃复温后过滤的红细胞悬液其溶血率存在显著性差异(P<0.05)。结论:现用的白细胞滤器能引起一定程度的红细胞溶血,红细胞悬液经35℃复温10m in后再行过滤可有效减少过滤引起的溶血。更多还原

【Abstract】 Objective To investigate the effects of hemolysis in different temperature during the removal of white blood cell and to approach the protection of red blood cell from hemolysis.Methods After storage for 5 days,40 units of red blood cell suspension were randomly divided,in equal units,into two groups,control group and test group.Control group was filtrated immediately after taking the blood out of refrigerator,while test group was incubated at 35℃ for 10 minutes before filtration.The plasma concentrations of potassium and free hemoglobin of all 40 units of red blood cell suspension were measured respectively before and after filtration.The results of hemolysis were analyzed which were measured with eyeballing in plasma of 650 units of red blood cell suspension after stored for 5 days(including 220 units of red blood cell suspension filtrated immediately after stored at 4℃ and 430 units of red blood cell suspension incubated at 35℃ for 10 minutes before filtration).Results The plasma concentrations of potassium and free hemoglobin in control group after filtration were(6.41±0.16)mmol/L and(37.84±6.15)mg/L,which were both significantly higher than those before filtration [(5.62±0.15)mmol/L,(23.31±4.24)mg/L](P<0.05);and in test group,they were(5.72±0.16)mmol/L,(27.93±5.28)mg/L after filtration,which had no significant difference with those before filtration [(5.63±0.15)mmol/L,(23.51±4.26)mg/L](P>0.05),and were significantly lower than those after filtration in control group(P<0.05).There was no significant difference in the plasma concentrations of potassium and free hemoglobin of the two groups before filtration(P>0.05).The rates of hemolysis had significant difference between two groups(P<(0.05)).Conclusion Removal of white blood cell with current filter can result in some degree in hemolysis.Incubating at 35℃ for 10 minutes before filtration can effectively reduce hemolysis induced by filtration.更多还原