【摘要】 目的:建立差式酚硫法并与酚硫法、蒽酮法、咔唑法一起比较测定五子衍宗颗粒剂中总多糖的含量;方法:先在酚硫法的基础上创立差示酚硫法,再与酚硫法、蒽酮法、咔唑法一并测定;结果:差式酚硫法的测定条件:样品可直接用未精制多糖水提液;反应在4.5h以内完成;测定波长λm ax=490nm;线性范围在3.56μg~35.6μg;相关系数r=0.996;RSD=2.85%;回收率为99.9%。用差式酚硫法、酚硫法、蒽酮法、咔唑法分别测得五子衍宗颗粒剂中多糖为(13.1±0.458)%,(9.97±0.416)%,(10.3±0.422)%,(10.3±0.610)%,经t检验,四法测定五子衍宗颗粒剂中多糖含量有显著性差异;结论:差式酚硫法可用于中药制剂多糖的含量测定,多糖可直接采未精制样品,不必分离纯化,因此该法操作简捷、快速、精密度高。更多还原

【Abstract】 Objective: To establish differential phenol sulfuric method(DPSM) and comparative analysis of determination of polysaccharides in Wuziyanzhang Granule with DPSM,phenol sulfuric method(PSM),authrone method(AM),carbazole method(CM);Method: The DPSM was set up basing on PSM.The determination of polysaccharides was carried out with DPSM,PSM,AM,CM;Result The DPSM was carried out under conditions that the sample was prepared with impure aquiferous solution;the operation was completed within 4.5h;The calibration curve was linear in the range from 3.56 to 35.μg,with r=0.996;The average recovery is 99.9%%;RSD is 2.85%;The Polysaccharides in Wuziyanzhang Granule was determined with DPSM,PSM,AM,CM as quantities were respectively(13.1±0.458)%,(9.97±0.416)%,(10.3±0.422)%,(10.3±0.610)%.The results show the four method,determined Polysaccharides in Wuziyanzhang Granule was significance different by t test.Conclusions: The DPSM is rapid,simple and precision to use in determination of Polysaccharides in the materials and preparations of pharmaceutics that was impure aquiferous solution.更多还原