【摘要】 【目的】观察云克(99Tc-MDP)对Ⅱ型胶原诱导性关节炎(CIA)大鼠的治疗作用及对血清IL-1β的影响。【方法】建立CIA大鼠关节炎模型,分为正常对照组,模型对照组,云克组,甲氨蝶呤组,后三组分别予以生理盐水,云克和甲氨蝶呤干预。采用关节炎指数评分法评价其关节的炎症程度;观察关节病理组织形态学变化并评分;放射酶联免疫法测定血清IL-1β水平。【结果】①关节炎指数评分:模型组、甲氨蝶呤组、云克组大鼠随着免疫时间延长,关节炎指数评分增加。36 d后模型组持续高于云克组和甲氨蝶呤组(P<0.05);44 d后甲氨蝶呤组高于云克组(P<0.05)。②组织病理学评分:模型组关节组织学评分均高于甲氨蝶呤组和云克组(P<0.05);炎性细胞渗出评分,云克组与甲氨蝶呤组无显著差异(P>0.05);软骨破坏和骨质破坏评分,云克组低于甲氨蝶呤组(P<0.05)。③模型组鼠血清IL-1β水平均明显高于正常组(P<0.01);模型组血清IL-1β水平明显高于甲氨蝶呤组、云克组(P<0.05);甲氨蝶呤组血清IL-1β水平高于云克组(P<0.05)。【结论】①云克明显减轻CIA大鼠的关节炎症状,延缓关节破坏的进展。②云克降低血清炎症细胞因子IL-1β的水平,从而阻止关节的破坏,可能是其治疗类风湿关节炎的机制之一。③在延缓CIA大鼠软骨和骨质破坏的病理学改变及降低IL-1β含量方面,云克的近期疗效均优于甲氨蝶呤。更多还原

【Abstract】 【Objective】To observe the treatment of the ⁹⁹Tc-MDP on type Ⅱ collagen induced arthritis（CIA） in rat,and the influence on the serum interleukin-1β（IL-1β）.【Methods】The CIA was established by subcutaneous injection with type Ⅱ collagen and incomplete Freud’s adjuvant to rats,except the normal group（n=6）.The rats successfully induced CIA（n=24） were divided into three groups randomly: ①Model group（n=8,treated with normal saline）;②⁹⁹Tc-MDP group(n=8,treated with ⁹⁹Tc-MDP);③Methotrexate group（n=8,treated with methotrexate）.The symptoms of arthritis were evaluated by arthritis index;the histopathological changes of joint were scored in infiltration of inflammatory cells,cartilage destruction and bone erosion;the serum levels of IL-1β were tested with radioimmunologic assay.【Results】①Arthritis index: The arthritis indexes of model group,methotrexate group,⁹⁹Tc-MDP group were increasing with the time elongation.The arthritis indexes of model group became higher than those of other two groups after 36 days（P<0.05）.When it came to the 44th day,the arthritis indexes of methotrexate group began to exceed those of ⁹⁹Tc-MDP group（P<0.05）.②The histopathological score: The histopathological scores of model control group were significantly higher than those of methotrexate group and ⁹⁹Tc-MDP group（P<0.01）.There were no significant differences between methotrexate group and ⁹⁹Tc-MDP group on infiltration of inflammatory cells（P>0.05）.The histopathological scores of cartilage destruction and bone erosion of ⁹⁹Tc-MDP group were lower than those of methotrexate group（P<0.05）.③Radioimmunologic assay: The serum levels of IL-1β in model group,⁹⁹Tc-MDP group and methotrexate group were notably higher than those of normal group（P<0.01）.Furthermore,the serum levels of IL-1β in model group were notably higher than those of ⁹⁹Tc-MDP group and methotrexate group（P<0.05）.The serum levels of IL-1β of ⁹⁹Tc-MDP group were notably lower than those of the methotrexate group（P<0.05）.【Conclusions】①⁹⁹Tc-MDP can notably relieve the arthritis symdrome of CIA in rats,and retard the catilage damage and bone erosion assessed by histopathology.②⁹⁹Tc-MDP could significantly decrease the serum levels of IL-1β.It might be one of the therapeutic mechanisms of ⁹⁹Tc-MDP.③⁹⁹Tc-MDP is better than methotrexate in retarding cartilage damage and bone erosion and in decreasing the serum levels of IL-1β of CIA rats in a 3-week therapeutic intervention.更多还原