【摘要】 目的:探讨大肠癌组织中Survivin和P53基因的表达及其与血管生成的关系。方法:采用免疫组化SP法,检测51例大肠癌(直肠癌22例,结肠癌29例;Dukes分期:A期13例,B期14例,C期15例,D期9例;高分化16例,中分化25例,低分化10例)组织中Survivin、P53的表达及微血管密度(MVD)。结果:Survivin、P53的表达与大肠癌的部位无关(P>0.05),而与组织分化程度、Dukes分期相关(P<0.05)。Survivin、P53阳性的大肠癌组织MVD(36.56±7.81,35.44±10.42)明显高于阴性者(19.81±9.27,22.17±9.54)(P<0.05)。Survivin、P53均为阳性时MVD值最高(39.78±7.88),高于其他各组(P<0.05)。Survivin与P53的表达呈正相关(r=0.957,P<0.05),MVD与Survivin、P53同时阳性表达呈正相关(r=0.787,P<0.05)。结论:Survivin和P53在大肠癌的发生和发展中起重要作用,并与肿瘤血管生成有关。更多还原

【Abstract】 Aim: To investigate the expression of Survivin and p53 gene and their relations with tumor angiogenesis in colorectal carcinoma tissue. Methods: Immunohistochemical SP method was used to detect the expression of Survivin and P53 and microvessel density (MVD) in 51 cases of colorectal carcinoma. Results: The positive expressions of Survivin and P53 were correlated with histologic differentiation and Dukes stage(P<0.05). The MVD in subgroups of survivin(+)(36.56±7.81) and P53(+)(35.44±10.42) were significantly higher than those in Survivin(-)(19.81±9.27)and P53(-)(22.17±9.54)(P<0.05),respectively. MVD in the subgroup of both Survivin(+)and P53(+)(39.78± 7.88)was significantly higher than those in the other subgroups (P<0.05). Survivin expression was positively correlated with P53 expression(r=0.957,P<0.05), and MVD was correlated with both Survivin and P53 expression(r=0.787, P<0.05). Conclusion: Survivin and P53 gene play a key role in the development of colorectal carcinoma, and correlate with the tumor angiogenesis.更多还原