【摘要】 目的:观察重组改构人肿瘤坏死因子α(rmhTNF-α)联合顺铂(CDDP)对人胶质瘤细胞生长的抑制作用。方法:人胶质瘤U-251细胞分为对照组(不加药);T组:加入rmhTNF-α(20μg/L);C组:加入CDDP(2mg/L);T+C组:加入rmhTNF-α(20μg/L)及CDDP(2mg/L)。MTT法检测细胞生长抑制率,用流式细胞仪检测细胞凋亡率及细胞周期变化情况,用免疫组织化学方法检测细胞PCNA、Bcl-2、P170蛋白的表达情况。结果:T组、C组细胞生长抑制率、细胞凋亡率均高于对照组(P<0.05),T+C组细胞生长抑制率、细胞凋亡率均高于其他各组(P<0.05)。T组PCNA-LI、Bcl-2蛋白表达低于对照组(P<0.05),T+C组PCNA-LI、Bcl-2蛋白表达均明显低于其他各组(P<0.05),对照组、T组及T+C组P170蛋白表达低于C组(P<0.05)。结论:rmhTNF-α能抑制人胶质瘤细胞生长,其机制与诱导细胞凋亡、抑制细胞增殖活性有关,且rmhTNF-α与CDDP联用有协同增效作用。更多还原

【Abstract】 Aim: To evaluate the effect of recombinant mutant human necrosis factor (rmhTNF) combined with cisplatin on the human glioma cell U251. Methods:U251 cells were divided into control group, rmhTNF-α (20 μg/L) treatment group, cisplatin (2 mg/L) treatment group and rmhTNF-α(20 μg/L)+cisplatin (2 mg/L) treatment group. The growth inhibition rate of U251 using was detected by MTT. Apotosis and cell cycle were detected by flow cytometry. The expression of PCNA, Bcl-2, and P170 were determined using immunohistochemistry. Results: The apoptosis rate and inhibition rate of U251 cells in cisplatin treatment group and rmhTNF-α treatment group were significantly higher than those of control group (P<0.05). In cisplatin+rmhTNF-α treatment group, the above indexes were higher than those of other groups (P<0.05). In rmhTNF-α cisplatin treatment group, the levels of PCNA and Bcl-2 proteins were lower than those of control group (P<0.05). The levels of PCNA and Bcl-2 in cisplatin+rmhTNF-α treatment group were significantly lower than those of other groups (P<0.05).The level of P170 in cisplatin treatment group was higher than that of rmhTNF-α treatment group, rmhTNF-α+cisplatin treatment group, and control group (P<0.05).Conclusion:rmhTNF-α could inhibit the growth of human glioma, through inducing apoptosis of glioma cells, and inhibiting the cell proliferation rmhTNF-α combined with CDDP have synergistic effects.更多还原