【摘要】 目的:探讨IL-12对炎症性肠病患者外周血和肠黏膜固有层淋巴细胞激活和炎症介质分泌的影响。方法:收集并分离15例克罗恩病、21例溃疡性结肠炎和13例结肠镜检正常者(对照)外周血单个核细胞(PBMC)及肠黏膜固有层单个核细胞(LPMC),体外培养并用IL-12刺激,培养12h、24h、48h后,用流式细胞仪测PBMC或LPMCCD69表达情况;培养48h后,收集细胞培养上清液并用ELISA检测干扰素(IFN)-γ、IL-2和肿瘤坏死因子(TNF)-α的水平。结果:IL-12刺激后炎症性肠病患者外周血T细胞表达高水平的CD69。克罗恩病患者的PBMC和LPMCCD69表达水平及IFN-γ、IL-2和TNF-α分泌水平较溃疡性结肠炎和对照者明显增高(P<0.05)。结论:IL-12能直接刺激炎症性肠病患者外周血和肠黏膜固有层淋巴细胞激活,并产生大量促炎症介质。更多还原

【Abstract】 Aim: To analyze the effect of IL-12 on peripheral blood and lamina propria lymphocyte activation and proinflammatory cytokine secretion in patients with inflammatory bowel disease. Methods:Peripheral blood mononuclear cell(PBMC) and lamina propria mononuclear cells (LPMC), isolated from 15 patients with Crohn’s disease, 21 patients with ulcerative colitis, and 13 healthy individuals, were cultured and stimulated with anti-CD3 and IL-12. After 12 h,24 h and 48 h,the expression of CD69 on PBMC and LPMC were detected using FCM. The supernatants were harvested after 48 h culture, and interferon (IFN)-γ, IL-2, and tumor necrosis factor (TNF)-α were assessed using ELISA.Results: The expression of CD69 on PBMC were in creased after IL-12 stimulation. The expression of CD69 on PBMC and LPMC from Crohn’s disease induced by IL-12, and IFN-γ, IL-2 and TNF-α secretion were higher compared with those in ulcerative colitis and normal controls (P<0.05).Conclusion: IL-12 directly stimulates activation of PBMC, LPMC and proinflammatory cytokine secretion from patients with inflammatory bowel disease.更多还原