【摘要】 目的:应用现代生物技术研究中国粗榧的组织培养技术。方法:观察不同消毒时间(5min,10min,15min),不同激素(6苄基嘌呤(6BA)、萘乙酸(NAA)、6呋喃氨基嘌呤(KT)、2,4二氯苯氧乙酸(2,4D))组合及不同激素浓度的MS培养基对中国粗榧不同的外植体材料(茎尖、当年生幼茎、当年生幼叶)愈伤组织诱导的影响。结果与结论:选用茎尖、幼茎为外植体,消毒时间为10min,培养基以MS+6BA+NAA+2,4D(6BA、NAA质量浓度均为20mg/L,2,4D为1.0mg/L)可较好地诱导中国粗榧愈伤组织的形成。更多还原

【Abstract】 Aim: To study the method of tissue culturing of Chinese cephalotaxus sinensis by modern biological technology.Methods: By observing the different sterilization time, the different hormone combination and the different explants, the optimum conditions of callus induction on Chinese cephalotaxus sinensis were studied. Results and Conclusion: It is shown that the stem apex and the young stem are suitable explants. With 10 min sterlization time and the culture medium MS+6-BA+NAA+2, 4-D, more ideal callus can be induced.更多还原