【摘要】 目的:探讨趋化因子CKLFSF8对人胃腺癌细胞株BGC823表皮生长因子受体(EGFR)表达的影响。方法:RTPCR检测细胞株BGC823中CKLFSF8基因的表达;利用脂质体将CKLFSF8转染BGC823;倒置显微镜观察BGC823生长情况,免疫细胞化学法检测CKLFSF8基因转染前后BGC823细胞EGFR的表达。结果:BGC823生长过程中可测到CKLFSF8表达;CKLFSF8转染前后BGC823细胞株EGFR表达的阳性率比较差异有统计学意义(P<0.05)。结论:CKLFSF8基因转染对人胃癌细胞株BGC823EGFR的表达有明显抑制作用,可望在肿瘤治疗中发挥重要作用。更多还原

【Abstract】 Aim: To investigate the effect of CKLFSF8 on EGFR expression of human gastric tumor cell line BGC823.Methods: The expression of CKLFSF8 mRNA on BGC823 cell line was assayed by RT-PCR. The target gene was transfected into the cell by liposome, and the cell growth was observed under inverted microscope. The expression of EGFR on tumor cells was determined by immunocytochemical technique. Results:Expression of CKLFSF8 existed in BGC823 cell. After transfection, the expression of EGFR of tumor cells was inhibited(P<0.05). Conclusion: The result shows that the expression of EGFR of BGC823 cells can be inhibited by transfection of CKLFSF8.The novel chemokine may has a bright prospect in the treatment of tumor.更多还原