【摘要】 目的:制备人脐血来源的树突状细胞(DCs),用胶质瘤RNA诱导其成熟,并观察DCs诱导细胞毒T淋巴细胞(CTL)体外杀伤肿瘤细胞的活性。方法:以rhGM CSF、IL4诱导人脐血单个核细胞向DCs分化并鉴定。提取胶质瘤RNA与DCs混合,使DCs致敏,未致敏的DCs为对照组。观察细胞形态并检测致敏前后DCs的表型。将DCs与外周血T细胞共培养,以胶质瘤细胞为靶细胞,采用MTT比色法检测T细胞抗肿瘤活性差异。结果:人脐血单个核细胞于诱导第5d呈现典型的DCs形态;经rhGM CSF、IL4诱导培养后,CD86、CD11c、CD54、MHCⅠ、MHCⅡ类分子表达与培养前比较均明显增高(P<0.01)。负载抗原后,MHCⅠ和CD54表达与负载抗原前相比进一步增高(P<0.05)。以DCs诱导活化的T细胞与胶质瘤细胞共培养可使胶质瘤细胞的生长受到抑制,并最终导致其死亡;杀伤率与未负载抗原的对照组相比差异有统计学意义(P<0.01)。结论:人脐血单个核细胞在细胞因子的诱导下扩增并分化为DCs。经抗原致敏后,能增强淋巴细胞对相应肿瘤细胞的体外杀伤效应。更多还原

【Abstract】 Aim: To investigate the anti tumor effects of cytotoxic T lymphocyte (CTL) induced by dendritic cells sensitized by glioma RNA. Methods:The mononunclear cells collected from normal cord blood were cultured into DCs with rhGM-CSF and IL B24. The RNA of glioma cell was extracted and mixed with DCs to induce sensitized DCs, and the normal DCs were used as control.The morphology of DCs was observed and the phenotype was detected before and after the sensitiz ation. DCs were cultured with peripheral blood T cells, and the anti-tumor effects of T cells on glioma cells were observed using MTT assay. Results: Typical DCs were observed 5 d later in mononuclear cells from human cord blood. Higher level CD86, CD11c, CD54, MHC-Ⅰ and MHC-Ⅱ were detected after induction by GM-CSF, IL-4. The expression of MHC-Ⅰ and CD54 was increased after the sensitization (P<0.05). T cells activated by DCs inhibited growth of tumor cells, and resulted in their death. The tumoricidal rate of sensitized DCs was higher than that of normal DCs (P<0.01). Conclusion: Human cord blood can differentiate into DCs under the stimulation of cytokines. It is capable of enhancing the cytotoxic activity of lymphocytes from peripheral blood in vitro after stimulation by antigen.更多还原