【摘要】 目的:定量观察托吡酯(TPM)对戊四氮(PTZ)点燃大鼠脑电图(EEG)的影响。方法:72只成年健康雄性SD大鼠随机分为4组。Ⅰ组大鼠胃灌入生理盐水(10ml/kg)后1h腹腔注射10g/L PTZ生理盐水溶液(35mg/kg);Ⅱ和Ⅲ组大鼠腹腔注射PTZ(剂量同Ⅰ组)前1h分别按100mg/kg和40mg/kg胃灌注10g/L TPM生理盐水溶液;Ⅳ组大鼠按10ml/kg和3.5ml/kg分别胃灌注和腹腔注射生理盐水。各组每d给药1次。当Ⅰ组大鼠痫性发作行为达到Ⅲ级(2周)、Ⅴ级(4周)和Ⅴ级后2周(6周)时分别描记EEG。结果:4周时,Ⅰ组大鼠出现多量阵发性短程高幅棘波、尖波及其综合波,Ⅱ组大鼠出现散在中、高幅尖波及尖慢综合波,Ⅲ组大鼠偶见中、高幅尖波及尖慢综合波。20minEEG连续描记中,Ⅱ组大鼠出现的异常波数量多于Ⅲ组大鼠(P<0.01);Ⅱ组、Ⅲ组大鼠发作的潜伏期延长,持续时间缩短(P<0.05)。2周时Ⅰ、Ⅱ和Ⅲ组大鼠注射PTZ后总功率、δ、θ和α功率均增加(P<0.05或0.01),β功率无改变(P>0.05);4周时,除I组大鼠β功率改变外,Ⅰ、Ⅱ和Ⅲ组注射PTZ后总功率和其他各频段功率均增加(P<0.05或0.01)。6周时,Ⅰ组大鼠注射PTZ后总功率、δ、α和θ功率增加(P<0.05或0.01),β功率无变化(P>0.05);除Ⅱ组总功率增加外(P<0.01),Ⅱ、Ⅲ组大鼠其他各频段功率无变化(P>0.05)。结论:TPM可拮抗大鼠PTZ点燃,抑制EEG的癫痫样放电。更多还原

【Abstract】 Aim: To observe the effect of topiramate(TPM) on quantitative EEG(qEEG) of pentylenetetrazol (PTZ)-kindled rats.Methods: A total of 72 healthy male rats were randomly divided into 4 groups (n=18). The rats of group Ⅰ were given normal saline at 10 ml/kg by gavage and intraperitoneally injected 10 g/L PTZ normal saline (35 mg/kg) daily; the rats of groupⅡ and Ⅲ were given 10 g/L TPM normal saline at 100 mg/kg and 40 mg/kg,respectively by gavage at 1 h before 10 g/L PTZ normal saline injection (35 mg/kg) daily; the rats of group Ⅳ(control group) were injected the same amount of normal saline as other groups. When the spasm in group Ⅰreached grade Ⅲ(2 weeks),V(4 weeks) and 6 weeks, qEEG was recorded and analyzed.Results: Group Ⅰ reached kindling criterion at 4 weeks. EEG was recorded at 2,4,and 6 weeks. At 4 weeks, EEG of group Ⅰ showed more typical spike-and-waves than group Ⅱ, and group Ⅲ only showed spike-and-waves occasionally, EEG of control group showed no epileptiform discharge.The latency was longer and persistence time markedly shortened in group Ⅱ and group Ⅲ (P<0.05). At 2 weeks, total power,absolute delta,theta, and alpha power in 3 groups increased(P<0.05 or 0.01). In group Ⅰ,Ⅱ,and Ⅲ,except that beta power did not change(P>0.05),the other power all increased(P<0.05 or 0.01).At 4 weeks,all power increased in group Ⅰ, Ⅱ,and Ⅲ (P<0.05 or 0.01)except beta power in group Ⅰ (P>0.05).At 6 weeks,in group Ⅰ,total power, delta, theta,and alpha power increased(P<0.05 or 0.01), but beta power had no statistical difference(P>0.05), and total power in group Ⅱ increased(P<0.01). Conclusion: Topiramate can prevent the rat from being kindled and inhibit epileptiform discharge.更多还原