【摘要】 目的:探讨角质形成细胞(KC)凋亡与银屑病病情的相关性。方法:比色法检测30例银屑病患者皮损中活性半胱氨酸天冬氨酸特异性蛋白酶-3(caspase-3)的含量;TUNEL技术检测KC凋亡并计算凋亡指数(AI);采用银屑病皮损面积和严重性指数(PASI)及局部病变的严重程度评分对其中26例寻常型银屑病患者予以病情程度评分。结果:30例银屑病中,脓疱型银屑病AI高于寻常型银屑病(P<0.05);26例寻常型银屑病患者中,进行期患者AI、活性caspase-3的含量均显著高于静止期和消退期(P<0.05);而静止期和消退期患者之间差别无统计学意义(P>0.05);PASI评分与AI及活性caspase-3含量之间均无直线相关性(P>0.05);银屑病皮损局部病变的严重程度评分与AI、活性caspase-3含量均呈直线正相关(P<0.05)。结论:KC凋亡的程度与银屑病的不同型别、疾病的不同分期有关,与局部病变的严重程度有关,与银屑病病情严重性指数无关。更多还原

【Abstract】 Objective To investigate the correlation between the apoptosis of psoriatric keratinocytes (KCs) in the psoriatic lesions and patient’s condition. Methods Thirty patients with psoriasis were included. Activated caspase-3 was examined by colorimetric method in skin biopsy specimens. The apoptotic KCs were detected with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay in skin biopsy specimens. The severities of 26 patients with psoriasis vulgaris (PV) were assessed using the PASI scoring system and the severities of psoriatic lesions. Results Apoptotic indexes (AI) were higher in the pustular psoriatic lesions than those in the vulgaris psoriatic lesions (P<0.05). In 26 patients with PV, AI and activated caspase-3 in the lesions were significantly higher in the progressive stage than those in the static and the regressive stages (P< 0.05) . There were no correlations between PASI and AI or the expression of activated caspase-3 in PV lesions (P>0.05). The severities of psoriatic lesions were positively correlated to AI or the expression of activated caspase-3 in PV lesions (P<0.05). Conclusion The amount of apoptotic KCs may be related to varied types, stages of psoriasis, and severities of psoriatic lesions.更多还原