【摘要】 目的:检测膝关节滑液(synovialfluid,SF)中细胞因子白介素-18(interleukin-18,IL-18)和炎症介质前列腺素E2(prostaglandinE2,PGE2)含量,探讨IL-18和PGE2在骨关节炎(osteoarthritis,OA)发病机制中的作用。方法:提取54例膝OA患者(OA组)的关节滑液,9例对照组(正常组)膝关节液,用ELISA酶联免疫吸附法和酶联免疫竞争法分别检测IL-18和PGE2含量,并对IL-18和PGE2两指标作线性相关与回归分析。结果:OA组膝关节滑液中IL-18和PGE2含量与正常组比较,有增高的趋势,差异有统计学意义(P<0.01)。对照组IL-18浓度值为(28.768±13.575)×10-9ng/L,OA组IL-18浓度值为(72.303±40.130)×10-9ng/L(P<0.01)。对照组PGE2浓度值为(24.697±7.814)×10-9ng/L,OA组PGE2浓度值为(42.302±23.818)×10-9ng/L(P<0.01)。分析表明IL-18与PGE2有较强正相关关系(对照组r=0.76,P<0.001;OA组r=0.94,P<0.001)。结论:本研究表明OA组关节滑液中IL-18与PGE2的含量明显高于对照组,说明IL-18与PGE2可能参与了骨关节炎的发病机制。IL-18的增高可能引起PGE2含量的增高,从而在OA的发病机制中发挥重要作用。更多还原

【Abstract】 Objective To detect the concentrations of interleukin-18 （IL-18） and prostaglandin E 2 （PGE 2 ） in synovial fluid （SF）, and to determine the role of IL-18 and PGE 2 in osteoarthritis（OA） pathogenesis. Methods IL-18 and PGE 2 were measured concurrently in synovial fluid samples from 54 patients with knee OA（OA group） and from 9 controls （control group）. Quantitative determination of IL-18 was performed by enzyme-linked immunosorbent assay （ELISA）. PGE 2 was examined by inhibitory enzyme-linked immunosorbent assay. A linear regression between IL-18 and PGE 2 was anaylsed. Results The concentrations of IL-18 and PGE 2 in SF from the OA group were significantly higher than those from the control group （P<0.01）. The average value of IL-18 in the control group was （28.768±13.575）×10 -9 ng/L, and （72.303±40.130）×10 -9 ng/L in the OA group （P< 0.01）; the average value of PGE 2 in the control group was （24.697±7.814）×10 -9 ng/L, and （42.302± 23.818）×10 -9 ng/L in the OA group （P<0.01）. IL-18 was related with PGE 2 in a linear curve fashion （the control group: r=0.76, P<0.001; the OA group: r=0.94,P< 0.001）. Conclusion IL-18 and PGE 2 are significantly higher in the OA group than those in the control group, and they might take part in the cartilage degradation in OA pathogenesis. The increase of IL-18 might induce the increase of PGE₂ , and that might play an important role in OA pathogenesis.更多还原